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Shanghai Biotechnology Co Ltd
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Sanko Junyaku Co Ltd
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Lonza
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Biotechnology Information
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Lonza
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China Center for Type Culture Collection
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Image Search Results
Journal: Toxicological Sciences
Article Title: Editor’s Highlight: Modeling Compound-Induced Fibrogenesis In Vitro Using Three-Dimensional Bioprinted Human Liver Tissues
doi: 10.1093/toxsci/kfw169
Figure Lengend Snippet: 3D bioprinted tissue exhibits a compartmentalized architecture and maintains hepatic stellate cells in a quiescent-like phenotype. (A) Illustration of a transverse cross-section of bioprinted tissue on a transwell insert comprising hepatocytes (HCs) and compartmentalized endothelial cells (ECs) and hepatic stellate cells (HSCs). (B) The organization of non-parenchymal cells (NPCs) is depicted with CD31 and vimentin staining to mark ECs and HSCs, respectively. Albumin is used to denote the hepatocellular compartment (HC). Scale bar = 100 µm, inset scale bar = 25 µm. (C) HSC activation status was examined using desmin (generic marker) and α-SMA (activation marker). Quiescent HSCs are denoted with white arrows. Scale bar = 50 µm.
Article Snippet: Three-dimensional bioprinted liver tissues were manufactured by Organovo (San Diego, California) using primary cryopreserved human HCs (Life Technologies, Carlsbad, California), HSCs (ScienCell, Carlsbad, California), and
Techniques: Staining, Activation Assay, Marker